He8atri7khiaShelljc He8atri7khiaShelljc

Today at 6:05 AM
Biology

Answered

Total nucleic acids are extracted from a culture of yeast cells and are then mixed with resin beads to which the polynulceotide 5'-TTTTTTTTTTTTTTTT-3' has been covalently attached. After a short incubation, the beads are then extracted from the mixture. When you analyze the cellular nucleic acids that have stuck to the beads, which of the following is most abundant?

a) DNA
b) tRNA
c) rRNA
d) mRNA

Answer :

JcAlmighty JcAlmighty

Today at 6:10 AM

The answer is d) mRNA

Transcription is a process of transcribing mRNA from DNA. At the end of the process, polyA tail (made of several adenines) is added to mRNA. So, mRNA deattaches and is able to undergo translation.
Resin beads have 5'-TTTTTTTTTTTTTTTT-3'.
Their complementary sequence would be: 3'-AAAAAAAAAAAAAAAA-5'.
So, the complementary sequence is actually the part of mRNA. Therefore, mRNA binds to the resin beads with attached polynucleotide sequence.

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