Answer :
The volume of the DNA solution, is 4μL, that needs to be added to the reaction mixture.
What is Dilution Equation?
The Dilution Equation is represented as
M₁V₁ = M₂V₂
where,
M₁ represent the Molarity of Stock solution
M₂ represent the Molarity of new solution
V₁ represent the initial volume from stock solution
V₂ represent the final volume from stock solution
Here
M₁ = 12.5 ng/μL
M₂ = 1 ng/μL
V₂ = 50 μL
Now put the values in above formula we get the initial volume as
M₁V₁ = M₂V₂
(12.5 ng/μL) x V₁ = (1 ng/μL) x 50 μL
12.5 x V₁ = 1 x 50 μL
V₁ = 50 / 12.5 μL
V₁ = 4 μL
Learn more about the Dilution Equation here: https://brainly.com/question/24709069
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NOTE: The given question is incomplete on the portal. Here is the complete question.
QUESTION: Let's say that you ordered 2.00 μg of double stranded DNA. You want to conduct an experiment but you need to prepare a DNA solution whose concentration is 12.5 ng DNA/1 μL of water. You then ordered forward (the amount being 234 μg) and reverse primers (the amount being 216 ug). You figure out that the molar mass of the forward primer is 6500 g/mol and that of the reverse primer is 6100 g/mol. You need to prepare a 100 μM solutions of both forward and reverse primers.
You then received an enzyme whose concentration is 5 units/μL. You also received a buffer that has a 200 mM Tris-HCl (pH 8.4), 500 mM KCl. They call this a 10X buffer (10 times the working concentration of the buffer). Working concentration is the concentration of Buffer when the PCR reaction is setup. Using all these solutions they plan to setup a new PCR reaction with a final reaction volume of 50 μL.
The final concentration of the DNA in the reaction mixture is 1 ng/ μL. Calculate the volume of the DNA solution (that was prepared earlier), in μL, that needs to be added to reaction mixture.