If the work is negative work, then the object will lose energy. The gain or loss in energy can be in the form of potential energy, kinetic energy, or both. Under such circumstances, the work that is done will be equal to the change in mechanical energy of the object.
"Kinetic energy is the energy of motion, observable as the movement of an object, particle, or set of particles." Any object in motion is using kinetic energy a person walking, a thrown baseball, a crumb falling from a table, and a charged particle in an electric field are all examples of kinetic energy at work.
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