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In glycolysis, the Six-carbon sugar glucose is split into_____ molecules of pyruvate.

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During glycolysis glucose is  converted to fructose  through rearrangement of its atoms. To this fructose are added two phosphate  molecules which becomes fructose-1,6 biphosphate because the phosphate molecules are attached at carbon 1 and 6  of the fructose.

This phosphorylated fructose molecule is then split into two 3-carbon molecules to form a couple of glucose- 3- phosphate (G3P) molecules, each of which gains another phosphate molecule.

Finally these two G3P molecules transfer their phosphate molecules to electron carriers and they each becomes a  pyruvate.

So the glucose molecule is split into two pyruvate molecules.